Jan 15, 2020 · The ratiometric electrochemical DNA biosensor was constructed and applied to the detection of exosomal miR-21. As shown in Fig. 1, firstly, a layer of PLLy was electropolymerized on the GCE surface, forming PLLy/GCE and providing abundant bonding sites for carboxyl.Next, a mixture solution of MB-Y1 modified with MB and amino group and Fc-Y2 modified with Fc and LNA was
An emerging interplay between extracellular vesicles and Feb 01, 2020 · Exosomes generation can occur in ESCRT-dependent or -independent manner using molecules and mechanisms that all enveloped viruses have hijacked from the infected cells to assembly and release viral particles . In this respect, enveloped viruses, and in particular retroviruses, have been proposed to be Trojan exosomes [, , ].
Request full-text PDF. Exosomes:Generation, structure, transport, biological activity, and diagnostic application and transport of exosomes, their structure and roles in normal conditions
Cathepsin D Is Secreted from M-BE Cells:Its Potential The early diagnosis of lung cancer is an effective approach to reduce the mortality caused by malignancy. To explore serum biomarkers of lung cancer at early stage, M-BE, a SV40T-transformed human bronchial epithelial cell line with the phenotypic features of early tumorigenesis at high passage, was cultured in the conditioned media to collect its secretory proteins. The proteins secreted from
Exosomal cargoes in OSCC:current findings and potential Oral squamous cell carcinoma (OSCC) is the most prevalent malignancy in head and neck cancer, with high recurrence and mortality. Early diagnosis and efficient therapeutic strategies are vital for the treatment of OSCC patients. Exosomes can be isolated from a broad range of different cell types, implicating them as important factors in the regulation of human physiological and pathological
The use of these carriers to transport therapeutic drugs is a recent and promising approach to treat diseases such as cancer and Alzheimer disease. The physiological production of these structures is limited impairing its collection and subsequent purification. These drawbacks inspired the search for mimetic alternatives.
Exosomes in virus-associated cancer - ScienceDirectDec 01, 2018 · Exosomes play an important role in the material transport and information exchange among cells, and they are closely related to the biological activities of living organisms. It is becoming clear that oncogenic viruses take advantage of exosomes to enhance viral pathogenesis.
From pathogenesis to clinical application:insights into Sep 29, 2016 · Exosomes are nanoscale extracellular membrane vesicles that are created by the fusion of an intracellular multivesicular body with the cell membrane. They are widely distributed in serum, urine, saliva and other biological fluids. As important transfer vectors for intercellular communication and genetic material, exosomes can stimulate target cells directly via receptor-mediated interactions
Microvesicles (MV) are membrane vesicles secreted from the plasma and endosomal membrane compartment by various cell types such as hematopoietic, epithelial, and tumor cells. Actively growing tumor cells shed MV, and the rate of shedding increases in malignant tumors. Although recent progress in this area has revealed that tumor-derived MV play multiple roles in tumor growth and metastasis via
Proteomic Analysis of Microvesicles Derived from Human Request full-text PDF. Exosomes:Generation, structure, transport, biological activity, and diagnostic application and transport of exosomes, their structure and roles in normal conditions
The Missing Link:How Exosomes and miRNAs can Help in Tamkovich S, Tutanov O, Laktionov P (2016) Exosomes:generation, structure, transport, biological activity, and diagnostic application. Biochem Moscow Suppl Ser A 10(3):163173. Article Google Scholar Tapia-Arancibia L, Rage F, Givalois L, Arancibia S (2004) Physiology of